Prof. Yuan He
Northwest University, China
Title: Remote Regulation of Enzyme-Nanozyme Cascade Reaction Kinetics by Magnetic Actuation
Abstract:
The remote regulation of enzyme activity is of great significance for both basic and applied research. Magnetic nanomaterials can generate a nanoscale magnetothermal effect under the exposure to an alternating magnetic field (AMF), which provides an opportunity for reversible and tunable control of enzymatic processes. Previously, our research group has built an in situ magnetothermal-spectroscopy platform to show enzyme immobilized on magnetic nanoparticles can be controlled by AMF in real-time. Here, we report a strategy for precise modulation of enzyme–nanozyme cascade reaction kinetics by remote magnetic stimulation. Glucose oxidase (GOx) was immobilized onto a ferrimagnetic vortex iron oxide nanoring (Fe3O4 NR) functionalized with PEG of different molecular weights to construct a series of Fe3O4 NR@GOx with nanometer linking distances. The activities of GOx and the Fe3O4 NR nanozyme in these systems were shown to be differentially stimulated by Fe3O4 NR-mediated local heat in response to an AMF, leading to modulated cascade reaction kinetics in a distance-dependent manner. Compared to the free GOx and Fe3O4 NR mixture, Fe3O4 NR(D2)@GOx with an optimum linking distance of 1 nm exhibits a superior kinetic match between GOx and the Fe3O4 NR nanozyme and over a 400-fold higher cascade activity under AMF exposure. This enables remarkable intracellular ROS production and significantly improved tumor inhibition in 4T1 tumor-bearing mice. The strategy reported here offers a straightforward new tool for fine-tuning multi-enzyme catalysis at the molecular level using magnetic stimuli and holds great promise for use in a variety of biotechnology and synthetic biology applications.
1. He, Y.; Chen, X.; Zhang, Y.; Wang, Y.; Cui, M.; Li, G.; Liu, X.; Fan, H., Magnetoresponsive nanozyme: magnetic stimulation on the nanozyme activity of iron oxide nanoparticles. Sci China Life Sci 2022, 65 (1), 184-192.
2. Xiong, R.; Zhang, W.; Zhang, Y.; Zhang, Y.; Chen, Y.; He, Y.; Fan, H., Remote and real time control of an FVIO-enzyme hybrid nanocatalyst using magnetic stimulation. Nanoscale 2019, 11 (39), 18081-18089.
3. Zhang, Y.; Wang, Y.; Zhou, Q.; Chen, X.; Jiao, W.; Li, G.; Peng, M.; Liu, X.; He, Y.; Fan, H., Precise regulation of enzyme-nanozyme cascade reaction kinetics by magnetic actuation toward efficient tumor therapy. ACS Appl Mater Interfaces 2021, 13 (44), 52395–52405.
Biography: